Phylogenetic relationships of D. rudis (Bedriaga, 1886) and D. bithynica(Mehely, 1909) based on microsatellite and mitochondrial DNA in Turkey

IF 1.1 4区生物学 Q4 GENETICS & HEREDITY

Mitochondrial Dna Part a Pub Date : 2017-11-01 DOI:10.1080/24701394.2016.1197215

Halime Koç, Bilal Kutrup, O. Eroğlu, U. Bülbül, Muammer Kurnaz, Feridun Afan, A. Eroğlu

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引用次数: 7

Abstract

Abstract The spiny-tailed lizard of the genus Darevskia have a series of taxonomic revisions, but still their phylogenetic relationships remain uncertain. In the present study, we have assessed taxonomic relationships among Darevskia bithynica and Darevskia rudis populations through estimation of phylogenetic relationships among 96 specimens using microsatellite DNA (Du215, Du281, Du323 and Du418 loci) and 53 specimens using mtDNA (16S rRNA and cytb) from main populations in Turkey. Although D. b. bithynica and D. r. mirabilis were separated based on the PCoA analysis at low level from other D. rudis and D. bithynica populations, the distance values of Nei's genetic distance, Nei's unbiased genetic distance, Fst and Linear Fst were not high among taxa in microsatellite DNA. On the other hand, our phylogenetic analyses (NJ, ML, MP and BI) did not separate D. rudis and D. bithynica populations. Finally, most of the topologically identical trees of phylogenetic analyses and microsatellite results showed that the extant populations of D. rudis and D. bithynica were found to be polytomy. Based on our molecular phylogenetic study, D. rudis complex is still ongoing revisions.

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土耳其D. rudis (Bedriaga, 1886)和D. bithynica(Mehely, 1909)基于微卫星和线粒体DNA的系统发育关系

刺尾蜥蜴属的分类学有一系列的修正，但它们的系统发育关系仍不确定。本研究利用微卫星DNA (Du215、Du281、Du323和Du418位点)和mtDNA (16S rRNA和cytb)对来自土耳其主要种群的96个标本和53个标本的系统发育关系进行了评估。微卫星DNA的Nei’s遗传距离、Nei’s无偏遗传距离、Fst和Linear Fst的距离值在各类群间均不高，但通过低水平的PCoA分析可将d.b.b bithynica和d.r.m arabilis与其他d.d.b bithynica居群分离出来。另一方面，我们的系统发育分析(NJ, ML, MP和BI)没有将d.r udis和d.b ithnica群体分开。最后，系统发育分析和微卫星结果显示，大部分拓扑结构相同的种群存在多聚性。基于我们的分子系统发育研究，D. rudis复合体仍在修订中。

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来源期刊

Mitochondrial Dna Part a Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.00

自引率

0.00%

发文量

期刊介绍： Mitochondrial DNA Part A publishes original high-quality manuscripts on physical, chemical, and biochemical aspects of mtDNA and proteins involved in mtDNA metabolism, and/or interactions. Manuscripts on cytosolic and extracellular mtDNA, and on dysfunction caused by alterations in mtDNA integrity as well as methodological papers detailing novel approaches for mtDNA manipulation in vitro and in vivo are welcome. Descriptive papers on DNA sequences from mitochondrial genomes, and also analytical papers in the areas of population genetics, phylogenetics and human evolution that use mitochondrial DNA as a source of evidence for studies will be considered for publication. The Journal also considers manuscripts that examine population genetic and systematic theory that specifically address the use of mitochondrial DNA sequences, as well as papers that discuss the utility of mitochondrial DNA information in medical studies and in human evolutionary biology.