Effect of Bacillus thuringiensis strains on growth and metabolic processes in Pisum sativum L. sprouts

Abstract

Although the entomopathogenic bacteria Bacillus thuringiensis Berliner are well-known bio-agents for regulating the population of leaf-eating pests of agricultural and ornamental crops, other agricultural properties of this microorganism are promising. In this work, the growth-stimulating effect of entomopathogenic strains of B. thuringiensis on Deviz pea plants was studied. The entomopathogenic strains B. thuringiensis 685, 926 and 109-C obtained from the Crimean Collection of Microorganisms of the Crimean Agricultural Research Institute, registered online (http://www.ckp-rf.ru) with number 507484, were used as research material. The morphometric parameters of pea sprouts were evaluated following standard methods. Biochemical parameters of pea sprouts were determined for 10-day-old sprouts. The amylase activity of pea sprouts was determined by photocalorimetry; total acidity was measured by titration using 0.1 n NaOH solution; total water-soluble phenolic compounds were determined by Leventhal titrimetric method. It was found that liquid spore culture of strains B. thuringiensis 685, 926 and 109-C had a stimulating effect on the length of root and stem and the weight of 10-day-old sprouts of Deviz pea variety. Treatment with a spore suspension of all tested strains B. thuringiensis led to an increase in the content of organic acids in the sprouts by an average of 12.4% compared with that of the control. Maximum stimulating effect on amylolytic activity and synthesis of phenolic compounds in Deviz pea sprouts was achieved by treating with a spore suspension of the strain B. thuringiensis 926. The amylase activity increased on average by 41.5% when compared to that of the control, while the total content of phenolic compounds in this experiment was 2.3 times higher than that of the control. Therefore, in light of the entomopathogenic properties and the obtained data on the growth-stimulating activity of the strains of B. thuringiensis, it can be concluded that these bacteria have additional potential for their use in agriculture as a bio-agent for plant protection having a complex action.