Antioxidant and Anti-inflammatory Activity of Salacca zalacca (Gaertn.) Voss Peel Ethanolic Extract on Lead Induced Fibroblast Cells

Abstract

: Lead toxicity is a serious environmental disease and its effects on the human body are overwhelming. Lead can increase reactive oxygen species (ROS) levels in the body which results in oxidative stress. Elevated ROS levels can stimulate inflammation and cellular aging. Plants extract have the abilities as antioxidant and anti-inflammatory agent to prevent aging and toxicity including Salacca zalacca peels extract (SPE). Cytotoxicity assay of SPE towards fibroblast cells (BJ) was handle using MTS (3-4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium). Intracellular ROS levels were observed by flow cytometry using DCF-DA fluorescent probe. Fibroblast cells were incubated at 37 0 C, 5% CO 2 , treated by 25 and 100 µg/ml SPE for 4 hours and followed by 400 µM Pb for 72 hours. Anti-inflammatory capacity was conducted using ELISA to measure IL-10 and TNF- α . SPE at 3.13-100 µg/ml were nontoxic to the BJ cells. Accumulation of intracellular ROS levels in lead-induced BJ cells were decreased by treatment using SPE at 25 and 100 µg/ml. SPE at 25 and 100 µg/ml elevated IL-10 and decreased TNF- α related to positive control (lead-induced cells). This research shows that S. zalacca peels extract has the ability as protective effect related to Pb poisoning.