BIODIVERSITY AND ANTIBIOTICS RESISTANCE OF OPPORTUNISTIC PATHOGENIC ENTEROBACTERIA ISOLATED FROM SURFACE RESERVOIRS OF ROSTOV-ON-DON

Abstract

Aim. Study biodiversity, sensitivity/resistance to antibacterial preparation (ABP) of opportunistic microorganisms of Enterobacteriaceae family isolated from surface reservoirs of Rostov-on-Don in 2014 - 2016. Materials and methods. Determination of genus and species membership of enterobacteria was carried out by the results of a complex of biochemical tests using MALDI Biotyper program-apparatus unit. Sensitivity to ABP was determined by serial dilution method. Results. 468 strains of opportunistic and pathogenic enterobacteria were isolated during the study. 20 genera, 33 species of microorganisms were identified. (37.0%), Klebsiella (13.2%), Citrobacter (8.0%) predominated. 8.5% of the strains isolated in 2014 were sensitive to ABP, 5.2% - in 2015; strains sensitive to ABP were not detected in 2016. The number of polyresistant isolates has increased during the last 3 years: 40.7% strains in 2014,60.1 % in 2015. 80,0% in 2016. Ceftriaxone, gentamycin and ciprofloxacin had high activity against the isolated enterobacteria. The highest resistance was documented against ampicillin, nalidixic acid, nitro-furane, co-trimoxazole. Conclusion. The data obtained allow to evaluate the dynamics of increase of polyresistant strains of enterobacteria isolated from surface reservoirs.