Evaluation of Formulated Anti-dermatophyte Creams from Ethanol Extract of Mitracarpus villosus Leaves

International Journal of Biochemistry Research and Review Pub Date : 2020-10-29 DOI:10.9734/ijbcrr/2020/v29i930219

A. Fawehinmi, F. Oyedeji

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引用次数: 1

Abstract

Microsporium, Trichophyton, and Epidermophyton are asexual fungi usually called dermatophytes. They are known to cause a kind of skin diseases called dermatophytosis. There have been reported cases of resistance by the dermatophytes with prolonged usage of synthetic antifungals on the skin. The aim of this study was to formulate herbal antifungal cream containing extract of Mitracarpus villosus as an anti-dermatophytic preparation and evaluate its physicochemical properties, stability and efficacy of the product. The formulated creams containing 0.5, 1 and 2% w/w of extract were subjected to stability tests using temperature variation method at -10, 4, 30, 37 and 45C. Freeze-thaw test, Centrifuge test, pH and exposure to UV light test were also carried out using standard method. Efficacy of the cream formulations were determined using Wistar rats as experimental animals. The percentage yield of the extract was (2.1%). Percentage ethanol phytochemical composition indicated that for alkaloid it is 1.06±0.04%, saponins (0.96±0.07%), flavonoids (0.06±0.02%) and tannins (0.04±0.01%). The emulsion produced was an oil-in-water emulsion and had a white colour with pH of 7.02, spread of emulsion, rubbing-in effect and stability to centrifugation was very high. The antifungal results showed the activity against the dermatophytes to be in the increasing order Original Research Article Fawehinmi and Oyedeji; IJBCRR, 29(9): 1-12, 2020; Article no.IJBCRR.61654 2 Epidermophyton floccosum (9 mm)

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长柄密枝叶乙醇提取物配方抗皮肤病膏的评价

小孢子菌、毛菌和表皮菌是无性真菌，通常称为皮菌。众所周知，它们会引起一种叫做皮肤真菌病的皮肤病。有报道的情况下，耐药的皮肤上长时间使用合成抗真菌剂的皮肤真菌。本研究的目的是制备含柔皮草提取物的抗真菌药膏，并对其理化性质、稳定性和药效进行评价。采用温度变化法在-10、4、30、37和45℃下对含有0.5、1和2% w/w提取物的配方面霜进行稳定性试验。采用标准方法进行冻融试验、离心试验、pH值试验和紫外光曝晒试验。以Wistar大鼠为实验动物，测定乳膏制剂的疗效。提取率为(2.1%)。生物碱(1.06±0.04%)、皂苷(0.96±0.07%)、黄酮类(0.06±0.02%)、单宁(0.04±0.01%)为乙醇含量。所制得的乳化液为水包油乳化液，呈白色，pH值为7.02，乳化液的扩散、摩擦效果和离心稳定性好。抑菌结果表明，其对皮肤真菌的抑菌活性呈递增趋势;生物工程学报，29(9):1-12,2020;文章no.IJBCRR。61654 2絮生表皮菌(9mm) < audounii小孢子菌(12mm) < mentagrophtes毛孢(13mm)

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International Journal of Biochemistry Research and Review

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