Protocol for Induction of Multiple Shoot through Nodal Explants Culture of Bambusa bambos for Biomass Production

Abstract

Aim of the present study was production biomass by induction of multiple shoots from Bambusa bambos. In general, the efficient and reproducible procedure for the propagation of bamboo can be achieved by seed propagation, clump division, and rhizome for small scale. In case of mass scale propagation, this technique would be highly insufficient and inefficient. For efficient production of bamboo, Micropropagation technique is used in large scale production. Nodal segment from fields grown clumps were used as the explants to develop a method of in vitro Micropropagation in bamboo. Plant growth hormone BAP (benzyl amino purine), KIN (kinetin), NAA (1naphthalene acetic acid), IBA (indole-3 butyric acid), IAA (indole-3 acetic acid) was studied on in-vitro Micropropagation of the effective shoot and roots of bamboo. Effective axillary bud breaking was achieved in Murashige and Skoog (MS) media. Nodal explants culture was inoculated in both solid (0.8%) and liquid MS media and observed the maximum proliferation of shoot in solid MS medium (4/ nodal explants). The concentration of sucrose was varied and their growth was examined. The sucrose was optimized (3%). Under the optimized sucrose condition, the hormone was varied and growth was examined. Under this condition, BAP response was high. Thus the concentration of BAP was varied for further studies. The response was high in 3 mg/l of BAP concentration. This review briefly provides the state-of-the-art information on tissue culture mediated biotechnologically interventions made in bamboo for large scale Micropropagation. The established protocol will be of help to stakeholders in edible bamboo trade to conserve genepool and increase productivity. Key-wordsBamboo, Micropropagation, Tissue culture, Multiple shoots, Benzyl amino purine INTRODUCTION Bamboo is a rhizomatous plant. It is a non-wood forestry product. It is one of the most important agriculture plants. It’s belonging to family Poaceae with woody culms growing uprightly. Bamboos assume a greater significance in the Indian context because after China, India has the second largest bamboo genetic resources in the world (23 genera and 125 species). The mass utilization of bamboo resources for hand craft industries, construction, paper and pulp industries, fishery, and human consumption. The biomass production is incomparable in bamboo plant. In the recent years, extensive research regarding micropropagation of bamboos has been done [1-3] . For carrying out in vitro propagation, different explants have been employed by Access this article online Quick Response Code Website: