Morphological characteristics and DNA barcoding in bach hop (Lilium poilanei Gagnep) in Vietnam

Abstract

The Lilium genus is a member of the Liliaceae family and is comprised of approximately 110 to 120 species. This genus is considered the largest genome with a haploid DNA content in the plant kingdom and is currently receiving much attention for its great commercial and trading prospects. Among them, Lilium poilanei belongs to the Sinomartagon group, plays an important role in the breeding of Asiatic hybrids. L. poilanei, widely known as an endemic plant in mountainous areas of Sapa, Vietnam and has been intensively exploited due to great commercial significance. In this study, the morphological characteristics and DNA sequence data including the nuclear ribosomal DNA segments of ITS, ITS2, and plastid matK, psbA-trnH and rbcL, rpoC1 were applied to identify L. poilanei collected. The results showed that some morphological details of the lily were specifically analyzed and both markers ITS2 and rpoC1 disclosed superiority in discrimination of L. polinanei with 100% similarity score by the one of ours reported in NCBI Genebank with the accession number of KR632775.1 and KR632777.1, respectively, while the remaining regions have been neither unamplified nor unsuccessfully discriminated and identified. Our findings in morphology and barcodes were reliable, effective and powerful for distinguishing L. poilanei and may identify other species of the family of Liliaceae.