Anticancer potential of Siddha formulations against oral cancer cell line in vitro

P. Ganapathy, K. Elumalai, M. Arumugam, Chinmayi Sri Amulya, R. Manivel
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引用次数: 3

Abstract

Treatment of cancer without any side-effects is still a challenge in the medical system. This leads to an increasing search for improved anticancer drugs. Plant products have been used as a traditional medicine for thousands of years as it has been drawing a great deal of attention to overcome cancer. The main objective of this study is to evaluate and compare the anticancer effect of MahaVallathy Leghiyam (MVL) and Neeradi Muthu Vallathy Leghiyam (NMVL) against human oral cancer (KB) cells. Different concentrations of aqueous extracts of MVL and NMVL were subjected to cytotoxic study. The antiproliferative effects were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and IC50 concentration was found at 3.25 mg/mL for MVL and 1.25 mg/mL for NMVL, also apoptotic activities were studied by PI and AO/EB dual staining. The results acquired from the comparative in-vitro studies on KB cell lines revealed that the unique Siddha medicine NMVL has more potent anticancer activity compared to MVL. There was an increase in the cell growth inhibition when treated with NMVL at lower concentration compared to MVL. The current investigation suggested that the phyto constituents of NMVL are responsible for anticancer activity. Thus, the long-term consumption of NMVL could be considered and promoted as an adjuvant therapy for treating various malignancies. *Correspondence to: Kayalvizhi Elumalai, Associate Professor, Department of Physiology, Meenakshi Medical College Hospital and Research Institute, Kanchipuram, Chennai–600 119, Tamil Nadu, India, Tel: 8870365116; E-mail: kayalgkbs@gmail.com Madan Kumar Arumugam, Scientist ‘C’, Cancer Biology Lab, Molecular and Nanomedicine Research Unit, Sathyabama Institute of Science and Technology, Tamil Nadu, India, Tel: 91 9942110146; E-mail: madankumarbio@gmail.com
悉达制剂对口腔癌细胞系的体外抗癌潜力
在医疗系统中,无副作用的癌症治疗仍然是一个挑战。这导致人们越来越多地寻找改进的抗癌药物。植物产品作为一种传统药物已经使用了数千年,因为它一直备受关注,以克服癌症。本研究的主要目的是评价和比较MahaVallathy Leghiyam (MVL)和Neeradi Muthu Vallathy Leghiyam (NMVL)对人口腔癌(KB)细胞的抗癌作用。对不同浓度的MVL和NMVL水提液进行细胞毒性研究。采用3-(4,5-二甲基噻唑-2-酰基)-2,5-二苯基溴化四唑(MTT)法测定其抗增殖作用,MVL的IC50浓度为3.25 mg/mL, NMVL为1.25 mg/mL,并采用PI和AO/EB双染色法研究其细胞凋亡活性。对KB细胞系的体外比较研究结果表明,独特的悉达药物NMVL比MVL具有更强的抗癌活性。与MVL相比,低浓度NMVL对细胞生长的抑制作用有所增加。目前的研究表明,NMVL的植物成分具有抗癌活性。因此,NMVL的长期使用可作为治疗各种恶性肿瘤的辅助治疗。*通信:Kayalvizhi Elumalai,副教授,Meenakshi医学院附属医院和研究所,坎奇普兰,金奈- 600 119,印度泰米尔纳德邦,电话:8870365116;Madan Kumar Arumugam,科学家' C ',癌症生物学实验室,分子和纳米医学研究单位,印度泰米尔纳德邦,Sathyabama科学技术研究所,电话:91 9942110146;电子邮件:madankumarbio@gmail.com
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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