Effect of certain additives on the quality of boar semen during preservation at 15°C and 5°C

Abstract

The study was aimed to evaluate the effect of four additives viz. KMnO4, Vitamin E, Butylatedhydroxytoluene and trehalose on quality of boar semen during preservation at 15°C and 5°C up to 96 hours. 24 ejaculates were utilized for the study. Sperm motility was significantly higher with BHT irrespective of preservation temperature and period. However, the mean sperm motility was significantly higher (P<0.05) in semen preserved at 15°C than 5°C. Live sperm was significantly (P<0.05) lower in Trehalose than KMNO4, Vitamin E and BHT. The mean percentage of live intact acrosome irrespective of temperature and preservation period was significantly higher (P<0.05) with BHT than others additives. Semen in Modena extender with BHT had significantly higher (P<0.05) percentage of HOST-reacted sperm at different preservation periods irrespective of different temperature. In conclusion, Butylatedhydroxyltoluene (BHT) was found to be superior to KMNO4, Vitamin E and Trehalose for preservation of Hampshire boar semen.