Qualitative Detection and Isolation of Bacteria from Surfaces of Canned Drinks Sold in Ugbor, Benin City

Ogofure G. Abraham, B. Idowu, Aduba U. Barbara, Ighodaro E. Veadams, E. O. Alexander
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引用次数: 6

Abstract

Abstract The qualitative assessment of putative bacterial pathogens on the surfaces of canned drinks sold in Benin metropolis was evaluated in this study. Standard bacteriological culture-based techniques employing the use of selective and differential media (Oxoid) such as Bacillus cereus agar, mannitol Salt agar, Pseudomonas cetrimide agar, bile esculin agar and MacConkey agar were used for isolation and identification of bacteria from swabbed surfaces of canned drinks. Kirby-Bauer disc diffusion technique was used for antibacterial susceptibility testing. The multiple antibiotic resistance (MAR) index was deduced from the antibiogram characterization to evaluate the public health importance of the bacterial isolates. Refrigerated samples had 25% contamination while 75% were not contaminated and about 15.39% contamination was observed for non-refrigerated samples (stored in crates or cartons) compared to the counterpart 84.61%. The bacterial species include Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Bacillus cereus, Bacillus sp. and Enterococcus sp. The bacteria were found to be sensitive to ciprofloxacin (92.5%) and gentamicin (90.1%) and least susceptible to cefixime (23.1%) and vancomycin (26.4%). They were found to be multi-resistant because they have an MAR index above the tolerable permissible limit (0.2) for common antibiotics usually used for their eradication. It is important to ensure that the surfaces of canned drinks must be rinsed with water before consumption.
贝宁乌格博尔市销售的罐装饮料表面细菌的定性检测与分离
摘要本研究对贝宁市销售的罐装饮料表面推定致病菌进行定性评价。采用选择性和差异培养基(Oxoid),如蜡样芽孢杆菌琼脂、甘露醇盐琼脂、假单胞菌琼脂、胆汁磷脂琼脂和麦康基琼脂等标准细菌学培养技术,从罐装饮料的拭子表面分离和鉴定细菌。采用Kirby-Bauer圆盘扩散法进行抗菌药敏试验。从抗生素谱特征推断多重抗生素耐药指数(MAR),以评估细菌分离株的公共卫生重要性。冷藏样品有25%的污染,75%没有污染,而非冷藏样品(储存在板条箱或纸箱中)的污染约为15.39%,而对应的污染为84.61%。细菌种类包括金黄色葡萄球菌、铜绿假单胞菌、大肠杆菌、蜡样芽孢杆菌、芽孢杆菌和肠球菌。细菌对环丙沙星(92.5%)和庆大霉素(90.1%)最敏感,对头孢克肟(23.1%)和万古霉素(26.4%)最不敏感。发现它们具有多重耐药,因为它们的MAR指数高于通常用于根除它们的普通抗生素的可容忍允许限度(0.2)。确保罐装饮料的表面在食用前必须用水冲洗,这一点很重要。
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