ANTIBACTERIAL ACTIVITY OF RAMBAI LEAF EXTRACT (Sonneratia caseolaris) CONCENTRATION 25% 50% 75% AND 100% AGAINST BACTERIA Enterococcus faecalis

Dentino Jurnal Kedokteran Gigi Pub Date : 2023-04-11 DOI:10.20527/dentino.v8i1.16076

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Abstract

ABSTRACTBackground: The causative factor of failure in the treatment of the root canal is the presence of microorganisms, namely the bacterium Enterococcus faecalis. Rambai leaf extract (Sonneratia caseolaris) also contains secondary metabolite compounds, namely, triterpenoids, phenols, tannins, steroids, and flavonoids that are able to inhibit the growth of Enterococcus faecalis bacteria which have the potential to cause failure in root canal treatment. Purpose: This research has a purpose to see the antibacterial activity of Rambai leaf extract (Sonneratia caseolaris) concentrations of 25%, 50%, 75%, and 100% against Enterococcus faecalis bacteria. Method: This study is a true experimental study designed by Posttest Only with Control Group Design using six. Material and methods: Rambai leaf extract concentrations of 25%, 50%, 75%, 100%, positive control of chlorhexidine gluconate 2%, and negative control of aqua dest was repeated 4 times. Antibacterial activity can be seen from the magnitude of the inhibitory zone formed in (MHA) Mueller Hinton Agar and BHIB (Brain Heart Infusion) using the diffusion method. Results: Based on Mann Whitney's final test, there was a significant difference in the clear zones formed around the paper disk. The average clear zone of Rambai leaf extract (Sonneratia caseolaris) concentration is 25% at 10.0mm, 50% at 12.7mm, 75% at 17.0mm, and 100% at 18.3mm. Conclusion: There is activity of Rambai leaf extract (Sonneratia caseolaris) concentrations of 25%, 50%, 75%, and 100% against Enterococcus faecalis bacteria. Keywords: Antibacterial Activity, Enterococcus faecalis, Rambai Leaf Extract

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兰白叶提取物浓度25%、50%、75%和100%对粪肠球菌的抑菌活性

摘要背景:微生物即粪肠球菌的存在是导致根管治疗失败的主要原因。兰白叶提取物(Sonneratia caseolaris)还含有次生代谢物化合物，即三萜、酚类、单宁、类固醇和类黄酮，它们能够抑制粪肠球菌细菌的生长，这些细菌有可能导致根管治疗失败。目的:考察25%、50%、75%、100%浓度兰白叶提取物对粪肠球菌的抑菌活性。方法:本研究采用后测法设计，采用对照组设计。材料与方法:兰白叶提取物浓度分别为25%、50%、75%、100%，阳性对照为葡萄糖酸氯己定2%，阴性对照为水溶液，重复4次。通过弥散法在(MHA) Mueller Hinton琼脂和BHIB (Brain Heart Infusion)中形成的抑菌带的大小可以看出抗菌活性。结果:根据Mann Whitney的最终测试，纸盘周围形成的清晰区有显著差异。蓝白叶提取物(Sonneratia caseolaris)浓度在10.0mm处平均为25%，12.7mm处为50%，17.0mm处为75%，18.3mm处为100%。结论:龙白叶提取物在25%、50%、75%和100%浓度下对粪肠球菌均有较强的抑菌活性。关键词:抗菌活性;粪肠球菌;兰白叶提取物

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Dentino Jurnal Kedokteran Gigi

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24 weeks