INHIBITORY ACTIVITY OF ULIN BARK (Eusideroxylon zwageri) EXTRACT TO Lactobacillus acidophilus

Dentino Jurnal Kedokteran Gigi Pub Date : 2021-11-10 DOI:10.20527/dentino.v6i2.11989

Ina Rezki Rahmasari, I. Firdaus, Renie Kumala Dewi

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Abstract

Background: Lactobacillus acidophilus is a bacterium which plays a role in dental caries. It is believed as a pioneering bacterium in advanced caries and much likely to be isolated in dentin caries zone, resulting in the needed for tooth restoration. The use of 2% Chlorhexidine digluconate as cavity cleanser is recommended as an effort to prevent seconday caries but can cause side effects. One of the natural materials that can be used as a cavity cleanser is ulin bark extract (Eusideroxylon zwageri), a traditional medicine originally from Kalimantan, because it contains phenolic, flavonoid, tannin, alkaloid, saponin and terpenoid. Purpose: To discover the inhibitory activity of ulin bark extract on Lactobacillus acidophilus growth. Methods: This was a true experimental laboratory and post test only with control group design, that used 20%, 40%, 60%, 80%, 100% concentrations of ulin bark extracts and K(+) 2% Chlorhexidine digluconate. Difussion method was used to test inhibitory activity with 6 treatment groups and 4 replications, comprising a total of 24 samples. All groups were incubated for 24 hours at 37ºC temperature. The inhibition zone was measured using calipers. Results: The 20%, 40%, 60%, 80% and 100% concentration of ulin bark extracts and 2% Chlorhexidine digluconate had an average inhibition zone of 7.17 mm, 9.02 mm, 11.14 mm, 13.06 mm, 15.17 mm and 19.22 mm. One Way ANOVA and Post Hoc Bonferroni tests showed significant difference between all groups. Conclusion: Ulin bark extract can inhibit Lactobacillus acidophilus growth starting from 20%, 40%, 60%, 80% and 100% concentration.

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乌林树皮提取物对嗜酸乳杆菌的抑制活性研究

背景:嗜酸乳杆菌是一种在龋齿中起重要作用的细菌。它被认为是晚期龋齿的先锋细菌，很可能在牙本质龋齿区被分离出来，从而导致牙齿修复的需要。建议使用2%的二光酸氯己定作为口腔清洁剂，以防止继发性龋齿，但可能会产生副作用。其中一种可以用作口腔清洁剂的天然材料是乌林树皮提取物(Eusideroxylon zwageri)，这是一种原产于加里曼丹的传统药物，因为它含有酚类、类黄酮、单宁、生物碱、皂苷和萜类。目的:研究乌林树皮提取物对嗜酸乳杆菌生长的抑制作用。方法:采用20%、40%、60%、80%、100%浓度的木质素树皮提取物和K(+) 2%的二光酸氯己定，采用真正的实验室和后验设计。采用扩散法检测抑制活性，6个处理组，4个重复，共24份样品。各组在37℃温度下孵育24 h。用卡尺测定缓蚀带。结果:20%、40%、60%、80%、100%浓度和2%二光酸氯己定的平均抑菌带分别为7.17 mm、9.02 mm、11.14 mm、13.06 mm、15.17 mm、19.22 mm。单因素方差分析和事后Bonferroni检验显示各组之间存在显著差异。结论:乌林树皮提取物在浓度为20%、40%、60%、80%和100%时均能抑制嗜酸乳杆菌的生长。

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Dentino Jurnal Kedokteran Gigi

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审稿时长

24 weeks