Surprising Effects of Rocking Motion on Leishmania tarentolae Behavior in Culture and Implications for Cell Stress

IF 2.6 3区 工程技术 Q2 MECHANICS
Linda M. Ferrence, Akshata Gajula, Marjorie A. Jones
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Abstract

Leishmania are an understudied genus of parasitic protozoans causing significant health problems for people, particularly in tropical climates. To better understand the growth of Leishmania and potential drug sensitivity implications, the effects of motion on cells grown in vitro were probed. Using a stock Leishmania tarentolae cell culture, cells were placed in 10 mL of a Brain–Heart Infusion medium in either a non-moving (static) environment or on a flat platform of one of two lab rockers (set at a minimal speed) in a dark environment for 13 days. Also, the addition of 0.5 M of L-Proline was evaluated. Microscopy, cell clumping, cell viability, and secreted acid phosphatase (SAP) activity data were collected. Results show that a constant slow rocking motion changed cell growth, clumping behavior, and detectable SAP activity relative to the no-motion cultures, but this change was dependent on which rocker was used, indicating a complexity in the growth of these cells in culture. Thus, continuous motion affects the stresses placed on the cells during a growth curve under some conditions. The implications of this study lead to questions about the effects of motion on the efficacy of pharmaceutical testing in vitro. Further study of the effects of motion on Leishmania is important.
摇摆运动对培养利什曼原虫行为的惊人影响及其对细胞应激的影响
利什曼原虫是一种未被充分研究的寄生原生动物属,对人类造成严重的健康问题,特别是在热带气候中。为了更好地了解利什曼原虫的生长和潜在的药物敏感性,研究了运动对体外生长的细胞的影响。使用原利什曼原虫细胞培养,将细胞置于10ml脑-心输注培养基中,在不移动(静态)环境中或在两个实验室摇臂之一的平坦平台上(以最低速度设置),在黑暗环境中放置13天。并对添加0.5 M l -脯氨酸进行了评价。显微镜、细胞结块、细胞活力和分泌的酸性磷酸酶(SAP)活性数据被收集。结果表明,相对于无运动培养,持续缓慢的摇摆运动改变了细胞的生长、结块行为和可检测的SAP活性,但这种变化取决于使用哪种摇杆,这表明这些细胞在培养中生长的复杂性。因此,在某些条件下,连续运动影响细胞在生长曲线上的应力。这项研究的意义导致了关于运动对体外药物测试功效的影响的问题。进一步研究运动对利什曼原虫的影响很重要。
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来源期刊
Journal of Thermal Stresses
Journal of Thermal Stresses 工程技术-力学
CiteScore
5.20
自引率
7.10%
发文量
58
审稿时长
3 months
期刊介绍: The first international journal devoted exclusively to the subject, Journal of Thermal Stresses publishes refereed articles on the theoretical and industrial applications of thermal stresses. Intended as a forum for those engaged in analytic as well as experimental research, this monthly journal includes papers on mathematical and practical applications. Emphasis is placed on new developments in thermoelasticity, thermoplasticity, and theory and applications of thermal stresses. Papers on experimental methods and on numerical methods, including finite element methods, are also published.
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