Evaluation of Anti-Cancer Effects of Caspian Cobra (Naja naja oxiana) Snake Venom in Comparison with Doxorubicin in HeLa Cancer Cell Line and Normal HFF Fibroblast

journal of ilam university of medical sciences Pub Date : 2021-12-01 DOI:10.52547/sjimu.29.6.20

Fatemeh Javani Jouni, Jaber Zafari, Elahe Shams, P. Abdolmaleki, A. Rastegari

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Abstract

most to evaluate the anti-cancer effects of Naja naja oxiana in comparison with doxorubicin in Hela (Human cervical cancer) and HFF (Human foreskin fibroblast) Material & Methods: Hela and normal fibroblast cancer cell lines were exposed to different concentrations (1, 10, 50, 100, and 500 μg/ml) of snake venom and doxorubicin. The MTT method was used to evaluate the IC50 (Inhibitory Concentration) for toxins and drugs. Finally, the results were analyzed using SPSS software (version 19). Findings: The results show that with increasing concentration and time of treatment with snake venom and doxorubicin, the percentage of Hela and fibroblasts living cells decreases. The highest decrease in the percentage of the viable cells was observed in the Hela cancer cell line treated with a concentration of 500 μg/ml snake venom for 48 h. Discussion & Conclusion: Snake venom can have a significant inhibitory effect on the percentage of living Hela cancer cells in comparison with doxorubicin.

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海眼镜蛇(Naja Naja xiana)蛇毒与阿霉素在HeLa癌细胞系和正常HFF成纤维细胞中的抗癌作用比较

材料与方法:将Hela和正常成纤维细胞癌细胞分别暴露于不同浓度(1、10、50、100和500 μg/ml)的蛇毒和阿霉素中，观察其对人宫颈癌和人包皮成纤维细胞的抗癌作用。采用MTT法测定毒素和药物的抑菌浓度(IC50)。最后，使用SPSS (version 19)软件对结果进行分析。结果显示，随着蛇毒和阿霉素处理浓度的增加和时间的延长，海拉细胞和成纤维细胞活细胞的比例降低。浓度为500 μg/ml的蛇毒作用于Hela癌细胞48 h后，其活细胞百分比下降幅度最大。讨论与结论:与阿霉素相比，蛇毒对Hela癌细胞的活细胞百分比有明显的抑制作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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journal of ilam university of medical sciences

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