The Correlation between Ferroptosis and m6A Methylation in Patients with Acute Kidney Injury

Kidney and Blood Pressure Research Pub Date : 2022-05-13 DOI:10.1159/000524900

Lihua Ni, Rui Bai, Qi-Lin Zhou, C. Yuan, Le-ting Zhou, Xiaoyan Wu

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引用次数: 8

Abstract

Objective: The present research analyzed the correlation between N6-methyladenosine (m6A) methylation and ferroptosis associated genes (FAGs) in acute kidney injury (AKI) patients. Methods: Bioinformatics analysis of microarray profiles (GSE30718) was performed to select differential expression genes (DEGs). FAGs are derived from systematic analysis of the aberrances and functional implications. The m6A methylation related genes were derived from the molecular characterization and clinical significance of m6A modulators. The multi-gene correlation of ferroptosis and M6A methylation modification was displayed. Then, the CIBERSORT algorithm was used to analyze the proportions of 22 immune cell infiltration. Results: In total, 349 DEGs were extracted between the AKI and control samples, among which 172 genes were upregulated and 177 were downregulated. FAGs (SLC1A5, CARS, SAT1, ACSL4, NFE2L2, TFRC, and MT1G) and m6A methylation related genes (YTHDF3, WTAP, and IGF2BP3) were significantly increased in AKI patients (p < 0.05). FAGs (SAT1, ACSL4, and NFE2L2) were positively correlated with the expression level of m6A methylation genes (p < 0.05). NFE2L2 has high diagnostic value, and the level of NFE2L2 was negatively correlated with the degree of follicular helper T (TFH) cell infiltration. Conclusion: Our research could provide a new theoretical basis for the pathogenesis and immune mechanism of AKI.

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急性肾损伤患者中铁下垂与m6A甲基化的关系

目的:分析急性肾损伤(AKI)患者n6 -甲基腺苷(m6A)甲基化与铁吊相关基因(FAGs)的相关性。方法:采用微阵列基因图谱(GSE30718)进行生物信息学分析，筛选差异表达基因(DEGs)。fag是通过对其异常和功能意义的系统分析而得出的。m6A甲基化相关基因来源于m6A调节剂的分子特征和临床意义。显示了铁下垂与M6A甲基化修饰的多基因相关性。然后，使用CIBERSORT算法分析22个免疫细胞浸润比例。结果:AKI与对照组共提取349个deg，其中上调172个，下调177个。AKI患者的FAGs (SLC1A5、CARS、SAT1、ACSL4、NFE2L2、TFRC、MT1G)和m6A甲基化相关基因(YTHDF3、WTAP、IGF2BP3)均显著升高(p < 0.05)。FAGs (SAT1、ACSL4、NFE2L2)与m6A甲基化基因表达水平呈正相关(p < 0.05)。NFE2L2具有较高的诊断价值，且NFE2L2水平与滤泡辅助T (TFH)细胞浸润程度呈负相关。结论:本研究为AKI的发病机制和免疫机制提供了新的理论依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Kidney and Blood Pressure Research

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