Partial Validation of Ultra Performance Liquid Chromatography Method for Quantification of Isoniazid-Pyrazinamide in Human Samples

The Journal of Pure and Applied Chemistry Research Pub Date : 2021-12-19 DOI:10.21776/ub.jpacr.2021.010.03.600

V. Yunivita, Triana Nurul Meirina, Atu Purnama Dewi, Harold Eka Atmaja, R. Ruslami

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Abstract

Isoniazid (INH) and pyrazinamide (PZA) are first-line drugs in the treatment of meningitis of tuberculosis, in addition to rifampicin. The use of these drugs will determine the success of therapy to kill Mycobacterium tuberculosis in brain tissue that is difficult to penetrate by other drugs. Therefore, it is necessary to conduct research and monitor the use of this drug in plasma and cerebrospinal fluid (CSF) patients with TBM. This study aimed to determine the method of analysis of INH and PZA using Ultra Performance Liquid Chromatography Ultra Violet (UPLC-UV). The samples were taken from TBM patients who taken INH-PZA and others besides TBM patients who did not take INH-PZA. The analytic method carried out includes a comparison of the results of the analysis method for measuring levels of standard INH-PZA and INH-PZA in plasma and CSF samples. Analysis of INH-PZA in plasma and CSF can be performed using UPLC with UV detector, at least 100 uL plasma or CSF samples volume, with reproducible and accurate results.

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超高效液相色谱法定量人样品中异烟肼-吡嗪酰胺的部分验证

除利福平外，异烟肼(INH)和吡嗪酰胺(PZA)是治疗结核性脑膜炎的一线药物。这些药物的使用将决定在其他药物难以穿透的脑组织中杀死结核分枝杆菌的治疗是否成功。因此，有必要对TBM患者血浆和脑脊液(CSF)中使用该药的情况进行研究和监测。本研究旨在建立超高效液相色谱-紫外(UPLC-UV)分析INH和PZA的方法。样本取自服用INH-PZA的TBM患者和其他未服用INH-PZA的TBM患者。所采用的分析方法包括将测定血浆和脑脊液样品中标准INH-PZA和INH-PZA水平的分析方法的结果进行比较。血浆和脑脊液中INH-PZA的分析可使用带UV检测器的UPLC进行，血浆或脑脊液样品体积至少为100 uL，结果重现性好，准确。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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The Journal of Pure and Applied Chemistry Research

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