Comparison of growth and toxicity responses between non-toxic and toxic strains of Prorocentrum hoffmannianum

IF 1.3 4区生物学 Q3 MARINE & FRESHWATER BIOLOGY

Aquatic Biology Pub Date : 2020-03-12 DOI:10.3354/ab00725

Thomas Chun-Hung Lee, P. Chan, Steven Jing-Liang Xu, Fred Wang-Fat Lee

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引用次数: 4

Abstract

We aimed to study the growth and toxicity responses of non-toxic (CCMP683) and toxic (CCMP2804) strains of Prorocentrum hoffmannianum under various nitrate and phosphate concentrations. The 2 strains were cultured in L1-Si medium with standard, depleted or 10-fold repleted nitrate or phosphate. CCMP683 cultured in standard L1-Si medium exhibited delayed growth. Nitrate or phosphate depletion decreased the cell density of both strains. Repletion of nitrate slightly increased the cell density of both strains. Repletion of phosphate also slightly increased the cell density of CCMP2804 but surprisingly decreased the cell density of CCMP683. Okadaic acid (OA) and its derivatives were not detected in CCMP683. OA was detected only in CCMP2804. Depletion of nitrate or phosphate increased the cellular concentration of OA, and repletion of nitrate or phosphate had no effect on the cellular concentration of OA. Correlation analysis indicated that the cellular concentration of OA was negatively correlated with cell density. Differences in the growth response to phosphate repletion and in the ability to produce OA suggest that the 2 strains may be good candidates for comparative studies related to phosphate metabolism and OA toxicity.

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霍夫曼原心菌无毒株与有毒株生长及毒性反应的比较

本实验旨在研究无毒菌株(CCMP683)和有毒菌株(CCMP2804)在不同硝酸盐和磷酸盐浓度下的生长和毒性反应。这2株菌株分别在标准、贫或10倍补充硝酸盐或磷酸盐的L1-Si培养基中培养。CCMP683在标准L1-Si培养基中生长迟缓。硝酸盐或磷酸盐的消耗降低了两种菌株的细胞密度。硝酸盐的补充使两个菌株的细胞密度略有增加。磷酸盐的补充也轻微增加了CCMP2804的细胞密度，但令人惊讶的是降低了CCMP683的细胞密度。在CCMP683中未检测到冈田酸及其衍生物。仅在CCMP2804中检测到OA。硝酸盐或磷酸盐的消耗增加了OA的细胞浓度，而硝酸盐或磷酸盐的补充对OA的细胞浓度没有影响。相关分析表明，OA细胞浓度与细胞密度呈负相关。在对磷酸盐补充的生长反应和产生OA的能力方面的差异表明，这两个菌株可能是与磷酸盐代谢和OA毒性相关的比较研究的良好候选者。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Aquatic Biology 生物-海洋与淡水生物学

CiteScore

2.70

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： AB publishes rigorously refereed and carefully selected Feature Articles, Research Articles, Reviews and Notes, as well as Comments/Reply Comments (for details see MEPS 228:1), Theme Sections, Opinion Pieces (previously called ''As I See It'') (for details consult the Guidelines for Authors) concerned with the biology, physiology, biochemistry and genetics (including the ’omics‘) of all aquatic organisms under laboratory and field conditions, and at all levels of organisation and investigation. Areas covered include: -Biological aspects of biota: Evolution and speciation; life histories; biodiversity, biogeography and phylogeography; population genetics; biological connectedness between marine and freshwater biota; paleobiology of aquatic environments; invasive species. -Biochemical and physiological aspects of aquatic life; synthesis and conversion of organic matter (mechanisms of auto- and heterotrophy, digestion, respiration, nutrition); thermo-, ion, osmo- and volume-regulation; stress and stress resistance; metabolism and energy budgets; non-genetic and genetic adaptation. -Species interactions: Environment–organism and organism–organism interrelationships; predation: defenses (physical and chemical); symbioses. -Molecular biology of aquatic life. -Behavior: Orientation in space and time; migrations; feeding and reproductive behavior; agonistic behavior. -Toxicology and water-quality effects on organisms; anthropogenic impacts on aquatic biota (e.g. pollution, fisheries); stream regulation and restoration. -Theoretical biology: mathematical modelling of biological processes and species interactions. -Methodology and equipment employed in aquatic biological research; underwater exploration and experimentation. -Exploitation of aquatic biota: Fisheries; cultivation of aquatic organisms: use, management, protection and conservation of living aquatic resources. -Reproduction and development in marine, brackish and freshwater organisms