COMPARATIVE ANTIMICROBIAL EFFECT OF ZEOLITES CONTAINING METALS ON STAPHYLOCCOCUS AUREUS, PSEUDOMONAS AERUGINOSA AND CANDIDA ALBICANS

Abstract

Pathogenic bioagents have a great influence on the health of animals and humans. Every year, the number of microorganisms that pose a threat to animal health is growing. Along with this, the large-scale use of antibiotics in agriculture creates problems of antibiotic resistance for pathogenic bioagents. The search for antimicrobials in this regard is a priority. As a result, the search for new antimicrobials, especially antibiotics, has become ubiquitous for animal health. In the world, scientists on this problem have published many works devoted to Staphyloccocus aureus, Pseudomonas aeruginosa, Candida albicans, etc. 200 ml of 0.1 N solutions of heavy metals (AgNO3, CuSO4, Ni(NO3)2, Pb(CH3COO)2, ZnSO4). Here we obtained a modified Ag, Cu, Ni, Pb and Zn zeolite tuff, which we tested on strains of the aforementioned pathogens. To ensure a constant concentration of metals in the experiment, the obtained dried powder was diluted in 10 ml of sterile distilled water and vigorously shaken before being placed on the surface of the medium with the applied pathogen culture. Among all synthetic zeolites tested on these three pathogens, zeolite containing Ag+ and Cu+2 exhibited antagonistic activity. A high antimicrobial effect of Ag zeolite was observed against P. aeruginosa and C. albicans compared to S. aureus. Conversely, copper-containing zeolite showed a higher antimicrobial effect against S. aureus with less effect on P. aeruginosa and almost no effect on C. albicans. Surprisingly, Ni-, Pb- and Zn-zeolites did not have any antagonistic effect on all tested pathogens. The control zeolite also had no effect on pathogens.