Expression of metallothionein in liver and kidney of freshwater fish Cyprinus carpio var. communis (Linn) exposed to arsenic trioxide

K. Kovendan, S. Vincent
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引用次数: 15

Abstract

In the present study, the toxic effects of arsenic trioxide (Ar2O3) to freshwater fish C. carpio were estimated at different median lethal concentration levels and its influences on the expression of metallothionein (MT) activity in liver and kidney were also observed. During this study, LC50 values were found to be 25.7, 27.4, 29.5, and 32 ppm for the period of 24, 48, 72, and 96 hrs respectively and LC16 and LC84 values of ArO3 also measured. The MT activity was estimated in liver and kidney of fish treated with different concentrations of ArO3 (5, 15, and 20 ppm) at different time intervals (48, 96, and 144 hrs) by using SDS-PAGE technique. From the results, 5 KD and 6 KD polypeptides were known to be metallothionein in liver and kidney respectively when the fishes were treated with different concentrations of arsenic arsenic trioxide. In the present investigation, ArO3 induced significant expression on metallothionein activity and it can be useful biomarker in environmental biomonitoring of arsenic contamination
三氧化二砷对淡水鱼鲤肝、肾金属硫蛋白表达的影响
本文研究了三氧化二砷(Ar2O3)在不同致死浓度下对淡水鱼C. carpio的毒性作用,并观察了其对肝脏和肾脏金属硫蛋白(MT)活性表达的影响。在本研究中,在24、48、72和96 h的时间内,LC50分别为25.7、27.4、29.5和32 ppm, ArO3的LC16和LC84也被测得。采用SDS-PAGE技术对不同浓度的ArO3(5、15和20 ppm)在不同时间间隔(48、96和144小时)处理的鱼肝脏和肾脏的MT活性进行了测定。结果表明,在不同浓度的三氧化二砷处理下,鱼的肝脏和肾脏中分别有5kd和6kd多肽为金属硫蛋白。在本研究中,ArO3诱导了金属硫蛋白活性的显著表达,可以作为砷污染环境生物监测的有用生物标志物
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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