The protective effects of polyherbal extracts of soybeans, coconut water, and brown rice (EMSA eritin) on stromal cell-derived factor 1 production in radiated mice

Abstract

The purpose of the present study was to provide the first evidence of the potential function of the polyherbal combination of soybeans, brown rice, and coconut water (EMSA eritin), as it has been suggested as a therapeutic agent because it, at least in part, increases the production of functional hematopoietic stem cells after irradiation. The study was conducted with 24 male mice (Mus musculus), which were divided into six groups consisting of four mice each. This study used three EMSA eritin doses: 1.04 mg/kg body weight (B.W.), 3.125 mg/kg B.W., and 9.375 mg/kg B.W. All treatment groups, except for normal controls, were exposed to sublethal doses of irradiation and then given EMSA eritin therapy for 15 days. Growth and differentiation of CD4 and CD8 T-lymphocytes in the thymus and the number of stromal cell-derived factor 1 (SDF1)-expressing cells in the spleen, bone marrow, and liver were analyzed using flow cytometry. Results showed that administration of EMSA eritin was capable of significantly inducing lymphocytic proliferation and differentiation and increased the number of SDF1-expressing cells in the spleen, bone marrow, and liver. SDF1 is a chemokine essential to lymphocyte migration and hematopoietic cell development; thus, it can be concluded that EMSA eritin is a good candidate as a therapeutic agent to restore the number of hematopoietic cells after irradiation.