A New Diagnostic Approach to Assessing Tissue Changes in Type 2 Diabetes Mellitus in Mice Using “LASMA ST” Device

Abstract

For the first time, LASMA ST, a device for laser diagnostic, was adapted for preclinical studies on laboratory db/db mouse genetic models of type 2 diabetes. The proposed method for studying of tissue changes during diabetes mellitus consists in a simultaneous control of microcirculation compartments: blood and lymph flow and oxidative coenzymes. The presented approach is characterized by a high informational value, safety and objectivity, as well as by the possibility of dynamic monitoring and obtaining online data on tissue metabolism (reduced nicotinamide adenine dinucleotide - NADH and oxidized flavin adenine dinucleotide - FAD).