Binary Transition Pattern (BTP) for Erythropoietic DNA Methylation Data

Abstract

Methods: Unlike the usual methods of selecting differentially methylated regions (DMR) by hypothesis statistical testing, we clustered CpG sites by their actual log2FC over time. Each cluster is represented by a 5-digit binary code. Results: We identified novel stage-specific TFBS during erythropoiesis. Our stage-specific differential methylation clusters have the ability to determine TFBS of co-regulators such as GATA1 and PU.1 at hypermethylated CpG loci.