Multidrug Resistance in Gram Negative Bacteria Isolated from Cases of Mastitis in Buffaloes

Abstract

: I n Egypt, buffaloes are considered as the second largest source of milk. Mastitis is a prime hindrance for milk production with severs economic losses, but scarce data are available about subclinical type in Egyptian buffaloes. The target of this study was to (a) Estimation of some biochemical alteration in mastitic whey milk and its role in milk quality and quantity. Mas-titis caused an increase in total whey protein, immunoglobulin and albumin in cows' milk samples with sub clinical mastitis, while α–lactalbumin, and β-lactoglobulin showed significant decreased. There was a highly significant decrease in α -casein and қ casein in sub clinical mastitic samples in comparing with the normal milk. In addition, there were a significant decrease in β-casein and non-significant decrease in γ casein milk protein fractions in sub clinical mastitic milk. (b) investigate the major gram-negative bacteria linked with buffalo’s mastitis, and (c) assess the antibiotic resistance pattern of the bacteria. To this end, 208 quarter milk samples were collected from 62 domesticated dairy buffaloes suffered from subclinical mastitis. Bacteriological assessment and biochemical methods, illustrated a total of 51 iso-lates (24.52%