A new method for obtaining metaphase chromosomes from the regeneration blastema of Penaeus (Marsupenaeus) japonicus

Abstract

Asimple technique for obtaining the well-spread metaphase chromosomes from the regeneration blastema of a prawn is described. The removed regeneration blastema of Penaeus(Marsupenneus)japonicus was treated with a slightly modified air-drying method. The diploid number of chromosomes of this prawn was 86.