A Novel Detection of Biomarker Molecule of α-synuclein for Parkinson Disease by Phospholipid Liposome-Immobilized Cantilever Biosensor Using Real-Time Quaking-Induced Conversion Method

Abstract

We newly applied real-time quaking-induced conversion (RT-QuIC) method for phospholipid liposome-immobilized cantilever sensor in order to obtain a trace amount of chronological behavior of α−synuclein (aSyn) as causative agent for Parkinson Disease. Our goal is to detect aggregated, not a monomeric, forms of aSyn in its initial stage, as it is most toxic in patient’s cerebrospinal fluid (CSF). Especially, by introducing similar processes of the RT-QuIC on a microscale cantilever surface, pM order aSyn fibril was detected, further indicating several hundreds of fM detection in the cantilever biosensor system that is fluorescent label-free technique.