Synthesis, in vitro Antibacterial and Cytotoxic studies of novel Naphthofurans

Abstract

A simple, convenient and reproducible naphthofuran derivatives (1-5) were synthesized from 1,4-naphthoquinones and characterized by 1 H-NMR, 13 C-NMR, FT-IR, and Mass spectral studies. All the newly synthesized compounds were evaluated for in vitro antibacterial activity against Staphylococcus aureus (ATCC 6538) , Staphylococcus epidermidis (ATCC 155) and Bacillus cereus (ATCC 11778) as Gram positive bacteria; Escherichia coli (ATCC 25922) , Klebsiella pneumonia (ATCC 29665) and Pseudomonas aeruginosa (ATCC 25619) as Gram negative bacteria and their minimal inhibitory concentrations were determined. Amongst the tested organisms, compound 1 was the most active compound against all the tested organisms. All the compounds were evaluated for in vitro cytotoxicity potential using the standard MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay against  human cervical cancer cell line (HeLa) and compound 3 showed more  potent than other compounds.