Cross-compatibility of Interspecific Cross in Tobacco

Abstract

Four wild species of tobacco(Nicotiana alata,N.rustica,N.repanda and N.stocktonnii)and four fluecured tobacco(K326,Hongda,Yun87,Yun97),were used as experimental materials in this research.The interspecific reciprocal crosses were carried out in order to study the compatibility.The seed setting percentage of cross was counted in field,and then test seed germination.Pollen tube behaviors were observed via fluorescence microscope after pollination.The results indicated that N.rustica,N.repanda and N.stocktonnii were incompatibility with the flue-cured tobacco.The pollen of N.rustica passed through the pistil of K326,but the pollens of N.repanda and N.stocktonnii were rarely growth on the pistil of K326.In spite of the pollen of N.alata passed through the pistil of K326,the hybrid tobacco seed had no vigor by the seed germination test.The female of N.alata was incompatibility with the flue-cured tobacco.