Genetic analysis of wild Ancherythroculter nigrocauda in tributaries and the main stream of the upper Yangtze River basin of China

IF 1.1 4区生物学 Q4 GENETICS & HEREDITY

Mitochondrial Dna Part a Pub Date : 2019-12-08 DOI:10.1080/24701394.2019.1698558

Taiming Yan, Xiongyan Wang, Song Li, Zhi He, Jie Luo, Qian Zhang, Deying Yang, Zhi He

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引用次数: 2

Abstract

Abstract Biodiversity is threatened by several factors that are often associated with overfishing, water pollution and hydroelectric dams, among other environmental impacts. The present study aimed to evaluate the genetic aspects of wild groups of Ancherythroculter nigrocauda using the mitochondrial cytochrome c oxidase subunit I (coI) and cytochrome b (cytb) genes and the d-loop region. We collected 89 representative individuals from three geographically distinct ranges of the Upper Yangtze River, including the Longxi River (LOR), Laixi River (LAR), and Hejiang range of the Yangtze River (HJ). The genetic analysis results showed that the three populations of A. nigrocauda had high levels of haplotype diversity (0.3434–0.951) and low levels of nucleotide diversity (0.00074–0.00412) based on the single gene sequences and the combination of gene sequences. Haplotype genealogy showed that only one haplotype (Hap-2) was shared by these three geographic groups, and 2–3 were shared by two groups; the other haplotypes were group-specific. The genetic distance within and between the populations was low; however, most of the molecular variance came from within the populations. Furthermore, high gene flow (>1.0) was found in HJ vs LOR and HJ vs LAR based on the d-loop region sequence and combination. These results suggested that there was a decrease in the degree of A. nigrocauda genetic diversity in the upper Yangtze River, and the genetic protection of the populations should be highlighted in the future.

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中国长江上游支流和干流野生黑斑斑鲷的遗传分析

生物多样性受到几个因素的威胁，这些因素通常与过度捕捞、水污染和水电站大坝以及其他环境影响有关。本研究旨在利用线粒体细胞色素c氧化酶亚基I (coI)和细胞色素b (cytb)基因和d-环区对黑绒螯蟹野生群的遗传方面进行评价。我们从长江上游的陇西、莱西和合江三个地理位置不同的河段收集了89个具有代表性的个体。遗传分析结果表明，3个居群单倍型多样性较高(0.3434 ~ 0.951)，核苷酸多样性较低(0.00074 ~ 0.00412)。单倍型家谱显示，3个地理类群共有1个单倍型(Hap-2)， 2个地理类群共有2-3个单倍型;其他单倍型是群体特异性的。居群内部和居群之间的遗传距离较低;然而，大多数分子变异来自群体内部。此外，基于d环区序列和组合，HJ vs LOR和HJ vs LAR的基因流量较高(>1.0)。这些结果表明，长江上游地区黑梭子蟹种群的遗传多样性呈下降趋势，今后应加强对黑梭子蟹种群的遗传保护。

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来源期刊

Mitochondrial Dna Part a Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.00

自引率

0.00%

发文量

期刊介绍： Mitochondrial DNA Part A publishes original high-quality manuscripts on physical, chemical, and biochemical aspects of mtDNA and proteins involved in mtDNA metabolism, and/or interactions. Manuscripts on cytosolic and extracellular mtDNA, and on dysfunction caused by alterations in mtDNA integrity as well as methodological papers detailing novel approaches for mtDNA manipulation in vitro and in vivo are welcome. Descriptive papers on DNA sequences from mitochondrial genomes, and also analytical papers in the areas of population genetics, phylogenetics and human evolution that use mitochondrial DNA as a source of evidence for studies will be considered for publication. The Journal also considers manuscripts that examine population genetic and systematic theory that specifically address the use of mitochondrial DNA sequences, as well as papers that discuss the utility of mitochondrial DNA information in medical studies and in human evolutionary biology.