Protective Effect of Tecoma stans Flowers on Gentamicin-Induced Nephrotoxocity in Rats

Abstract

The present study was undertaken to evaluate the Ethanolic Extract of Tecoma stans (EETS) flowers for its protective effect on gentamicin-induced nephrotoxocity in rats. Nephrotoxocity was induced in rats by intraperitoneal administration of gentamicin 80 mg kgG dayG for seven days. Effect of concurrent administration of EETS at a dose of 500 mg kgG dayG given by intraperitoneal route was determined using serum and urinary creatinine and blood urea nitrogen as indicators of kidney damage. As nephrotoxocity of gentamicin is known to involve the induction of oxidative stress, so in vitro antioxidant activity, malondialdehyde (MDA) and glutathione (GSH) levels were also evaluated. Gentamicin-induced glomerular congestion and necrosis of kidney cells were found to be reduced in the group receiving EETS with gentamicin. Rats treated with gentamicin developed significant kidney dysfunction was observed from increased levels of urea, creatinine, sodium and decreased levels of protein, uric acid and potassium. In vitro studies revealed that the EETS posses significant antioxidant activity. The above results confirm that the flower extract acquire nephroprotective and regenerative capacity against gentamicin toxicity.