Improved antimicrobial activity of Zinc-Oxide Nanoparticles in peritoneal dialysis fluid using Silk Fibroin Protein coating

Q2 Engineering

Materials Research Innovations Pub Date : 2022-12-13 DOI:10.1080/14328917.2022.2157984

Vandana Singh, Pranjali Pranjali, R. Raj, A. Guleria, Sachin Yadav, V. Kumar R, Dinesh Kumar

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引用次数: 1

Abstract

ABSTRACT Bacterial infections in the abdominal area are a primary cause of complications in patients with renal failure who are on peritoneal dialysis (PD). To combat such infections, Zinc Oxide (ZnO) Nanoparticles (NPs) are gaining popularity because of their well-known antibacterial characteristics. However, due to their quick accumulation and generation of bio-conjugates upon interaction with the components of PD Fluid, surface modification is required. In this context, the current study’s aim was to coat the ZnO-NPs with Silk Fibroin (SF) protein and assess their stability in PD fluid. UV-vis spectroscopy, Proton Nuclear Magnetic Resonance (1 H NMR) spectroscopy, Scanning electron microscopy (SEM) and Dynamic Light Scattering (DLS) characterisations, all validated the stability of SF-coated ZnO-NPs in PD fluid. Furthermore, SF-coated ZnO-NPs were tested as an antibacterial agent against Staphylococcus aureus, and our findings demonstrated that SF-coated ZnO-NPs significantly suppressed S. aureus activity.

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丝素蛋白包被改善腹膜透析液中氧化锌纳米颗粒的抗菌活性

腹部细菌感染是腹膜透析(PD)肾衰患者并发症的主要原因。为了对抗这种感染，氧化锌纳米颗粒(ZnO)因其众所周知的抗菌特性而越来越受欢迎。然而，由于它们在与PD流体的组分相互作用后迅速积累和产生生物偶联物，因此需要对其表面进行改性。在这种情况下，本研究的目的是用丝素蛋白(SF)包裹ZnO-NPs，并评估它们在PD液中的稳定性。紫外可见光谱、质子核磁共振(1h NMR)光谱、扫描电镜(SEM)和动态光散射(DLS)表征均验证了sf包覆ZnO-NPs在PD流体中的稳定性。此外，我们还测试了sf包被的ZnO-NPs作为金黄色葡萄球菌的抗菌药物，我们的研究结果表明，sf包被的ZnO-NPs显著抑制金黄色葡萄球菌的活性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Materials Research Innovations 工程技术-材料科学：综合

CiteScore

5.20

自引率

0.00%

发文量

审稿时长

2.8 months

期刊介绍： Materials Research Innovations covers all areas of materials research with a particular interest in synthesis, processing, and properties from the nanoscale to the microscale to the bulk. Coverage includes all classes of material – ceramics, metals, and polymers; semiconductors and other functional materials; organic and inorganic materials – alone or in combination as composites. Innovation in composition and processing to impart special properties to bulk materials and coatings, and for innovative applications in technology, represents a strong focus. The journal attempts to balance enduring themes of science and engineering with the innovation provided by such areas of research activity.