Genomic diversity between Radopholus similis populations from around the world detected by RAPD-PCR analysis.

Abstract

DNA extracts from thirteen populations of Radoplaolus similis, collected previously from roots of various hosts at different locations around the world, and one population each of R. bridgei and R. citri, both collected in Indonesia, were compared using the polymerase chain reaction based method of random amplified polymorphic DNA (RAPD-PCR). Hierarchical cluster analysis was applied to the data obtained from RAPD profiles. This arranged the fifteen populations into two clusters. It also highlighted the two populations of R. citri and R. bridgei which were genomically distinct from R. similis. The results are discussed in the context of the genomic divergence of the species R. similis and the genus Radopholus.