Apoptosis Induction with Enhancement of BAX/BCL2 Gene Expression Ratio via Combination Therapy in HT29 Colon Cancer Cells

Medical laboratory sciences Pub Date : 2020-12-08 DOI:10.22037/AMLS.V6.33489

Roya Bazzaz, P. Yaghmaei, S. Dastmalchi, N. Rashtchizadeh

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Abstract

Background and Aim: Combination therapy is one of the new strategies that minimize resistance to chemotherapy and reduces drug toxicity. Here, we investigated the effect of combination therapy with 5-Fluorouracil and Gamma Tocopherol on cell survival and BAX/BCL2 gene expression ratio in HT29 colon cancer cells. Methods: The proliferation of cancer cells was determined via colony formation assay. BAX/BCL2 ratio was evaluated after incubation with concentrations of 5-Fluorouracil and Gamma Tocopherol via real-time-PCR. Results: The average number of colonies in the cells treated with 5-Fluorouracil, Gamma Tocopherol and their combination of them was 63±4, 78±3, and 28±2, respectively which significantly decreased in the combination group. In contrast with the control group, the BAX/BCL2 ratio remarkably increased when the cell underwent combinational treatment (p<0.05). Conclusion: 5-Fluorouracil and Gamma Tocopherol reduced HT 29 cell proliferation. Our results suggest that combination therapy with 5- Fluorouracil and Gamma Tocopherol can be considered as a strategy for induction of apoptosis via increasing the BAX/BCL2 ratio. *Corresponding Author: Nadereh Rashtchizadeh; Email: rashtchizadeh@rocketmail.com Please cite this article as: Bazzaz R, Yaghmaei P, Dastmalchi S, Rashtchizadeh N. Apoptosis Induction with Enhancement of BAX/BCL2 Gene Expression Ratio via Combination Therapy in HT29 Colon Cancer Cells. Arch Med Lab Sci. 2020;6:1-7 (e21). https://doi.org/10.22037/amls.v6.33489

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联合治疗提高BAX/BCL2基因表达率诱导HT29结肠癌细胞凋亡

背景与目的:联合治疗是减少化疗耐药和降低药物毒性的新策略之一。我们研究了5-氟尿嘧啶和γ生育酚联合治疗对HT29结肠癌细胞存活和BAX/BCL2基因表达比的影响。方法:采用集落形成法测定癌细胞的增殖情况。用5-氟尿嘧啶和γ生育酚浓度孵育后，通过实时荧光定量pcr评估BAX/BCL2比值。结果:5-氟尿嘧啶、γ生育酚及其联合用药组细胞平均菌落数分别为63±4、78±3、28±2，联合用药组细胞平均菌落数明显减少。与对照组相比，联合处理时细胞BAX/BCL2比值显著升高(p<0.05)。结论:5-氟尿嘧啶和γ生育酚可抑制HT - 29细胞增殖。我们的研究结果表明，5-氟尿嘧啶和γ生育酚联合治疗可以被认为是通过增加BAX/BCL2比值来诱导细胞凋亡的策略。*通讯作者:Nadereh Rashtchizadeh;Bazzaz R, Yaghmaei P, Dastmalchi S, Rashtchizadeh N.联合治疗对HT29结肠癌细胞BAX/BCL2基因表达率的诱导作用。中华医学杂志，2020;6:1-7 (e21)。https://doi.org/10.22037/amls.v6.33489

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Medical laboratory sciences

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