Type I nitric oxide synthase in the human lung is predominantly expressed in capillary endothelial cells

Hardi Lührs , Thomas Papadopoulos , Harald H.H.W Schmidt , Thomas Menzel
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引用次数: 37

Abstract

Nitric oxide (NO) has important functions in the regulation of pulmonary smooth muscle tone. In the human lung, published data on the expression and distribution of neuronal nitric oxide synthase (NOS-I) are contradictory. The aim of this study, therefore, was to determine the predominant cells expressing NOS-I in the human lung. Immunofluorescence double staining techniques were applied to normal human lung tissue using established monospecific antibodies directed against NOS-I. Suprisingly, capillary endothelial cells in the alveolar septa were identified as the major sites of NOS-I expression. Neither alveolar nor bronchiolar epithelium, nor the alveolar macrophages, expressed NOS-I. These results indicate that the predominant sites of NOS-I expression in the human lung are confined to non-neuronal, i.e. capillary endothelial cells and suggest a role for NO in the regulation of pulmonary endothelial cell permeability.

人肺I型一氧化氮合酶主要在毛细血管内皮细胞中表达
一氧化氮(NO)在调节肺平滑肌张力中具有重要作用。在人肺中,已发表的关于神经元一氧化氮合酶(NOS-I)的表达和分布的数据是矛盾的。因此,本研究的目的是确定在人肺中表达NOS-I的优势细胞。利用已建立的针对NOS-I的单特异性抗体,将免疫荧光双染色技术应用于正常人肺组织。令人惊讶的是,肺泡间隔的毛细血管内皮细胞被确定为NOS-I表达的主要部位。肺泡上皮、细支气管上皮及肺泡巨噬细胞均未表达NOS-I。这些结果表明NO - i在人肺中的主要表达位点局限于非神经元细胞,即毛细血管内皮细胞,并提示NO在调节肺内皮细胞通透性中起作用。
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