Hydrolisis enzymatic protein from microsimbiont red algae Eucheuma cottonii as an antibacterial

Abstract

The research on the hydrolysis of protein from red algae has been carried out by microsymbionts E. cottonii as an antibacterial. This study aims to determine the effect of hydrolysis time on the degree of hydrolysis and antibacterial activity. The stages of this study include protein isolation, protein purification and protein hydrolysis. Protein hydrolysis using pepsin enzyme with 0.30,60,90 and 120-minute time variations.The final stage of this research is to test the antibacterial activity using the agar diffusion method with bioindicator bacteria S. aureus and E. coli. The results of this study indicate that the highest DH is 83.49% with a hydrolysis time of 90 minutes. Like wise, the largest inhibition zone for bioindicator bacteria was shown by the Ha4 hydrolyzate. The zone of inhibition against S. aureus is 21.1 mm, and E. coli is 14.8 mm.