Cytotoxic potentiality of Colocasia esculenta leaves extract on five different cancer cell lines using 3-(4,5-Dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide assay

R. Jyothi
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引用次数: 1

Abstract

Background: Medicinal plants have been playing a major role because of their therapeutic importance in terms of combating various diseases. Colocasia esculenta is an annual plant known for its medicinal properties. Aim: The present investigation was aimed to screen the cytotoxic potentiality of C. esculenta leaves extract against five different cancer cell lines. Materials and Methods: Ethanol was used as a solvent to extract plant material using hot extraction method. In our investigation, five different cancer cell lines such as human lung cancer (A549), ovarian cancer (Pa-1), prostate cancer (PC3), colon cancer (HCT 116), and acute leukemia (K562) were exposed to dose dependent cytotoxic studies. 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide assay carried out to determine the efficacy of ethanol extract of indigenous medicinal plant C. esculenta leaves in hindering the activity of cancerous cells and cisplatin was used as standard anticancer drug. Results: The inhibitory concentration (IC50) values recorded for Pa-1, A549, HCT116, K562, and PC-3 are 93.2 µg/mL, 133.6 µg/mL, 172.87 µg/mL, 217.54 µg/mL, and 223.08 µg/mL, respectively. The IC50 values ranged 93.2 µg/mL–223.08 µg/mL indicate the presence of variety of active principles present in the ethanolic extract. Conclusion: The ethanolic extract showed dose-dependent activity against all five different cancer cell lines. Investigation provides basic evidence to enlist C. esculenta leaves as one of the plants with cytotoxic properties, encourages to isolate bioactive compounds, and understands their interaction with cancer cells.
用3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四氮唑测定绿草叶提取物对5种不同癌细胞的细胞毒性
背景:药用植物在防治各种疾病方面发挥着重要的治疗作用。土芋是一种一年生植物,以其药用价值而闻名。目的:研究金钱树叶提取物对5种不同癌细胞的细胞毒作用。材料与方法:以乙醇为溶剂,采用热萃取法提取植物材料。在我们的研究中,五种不同的癌细胞系,如人类肺癌(A549)、卵巢癌(Pa-1)、前列腺癌(PC3)、结肠癌(HCT 116)和急性白血病(K562)暴露于剂量依赖性细胞毒性研究中。采用3-(4,5-二甲基噻唑-2基)-2,5-二苯基溴化四氮唑测定土生药用植物牛毛草叶乙醇提取物对癌细胞活性的抑制作用,并以顺铂为标准抗癌药物。结果:Pa-1、A549、HCT116、K562、PC-3的抑制浓度(IC50)分别为93.2µg/mL、133.6µg/mL、172.87µg/mL、217.54µg/mL、223.08µg/mL。IC50值范围为93.2µg/mL - 223.08µg/mL,表明乙醇提取物中存在多种活性成分。结论:乙醇提取物对5种不同的肿瘤细胞具有剂量依赖性。本研究提供了初步的证据,证明牛蒡叶是具有细胞毒性的植物之一,有助于分离出生物活性化合物,并了解其与癌细胞的相互作用。
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