In vitro anticancer activity of methanol extract of Cladophora spp. against HT-29 cell line

Abstract

The anticancer activity of the marine alga Cladophora spp. against HT-29 colon cancer cell line was studied. In the present investigation crude extract of seaweed was prepared by using soxhlet apparatus. Methanol was used as solvent for extraction. In vitro anticancer activity of seaweed at various concentrations (7.8 to 1000 μg/mL) were studied against the chosen cell line by using MTT assay (3-(4, 5-dimethyl thiazol 2)2, 5-diphenyl tetrazolium bromide, a yellow tertrazol). The most potent antitumor activity has been shown at the concentration 7.8 μg/mL and 400 μg/mL of Cladaphora spp. extract on HT-29 colon cancer cell line.