Time of Day and Length of Antidepressant Drug Administration Influence Brain-Derived Neurotrophic Factor and TrkB Levels in Rat Brain

Megan E. Kozisek, N. Qutna, D. Bylund
{"title":"Time of Day and Length of Antidepressant Drug Administration Influence Brain-Derived Neurotrophic Factor and TrkB Levels in Rat Brain","authors":"Megan E. Kozisek, N. Qutna, D. Bylund","doi":"10.2174/1874143600903010001","DOIUrl":null,"url":null,"abstract":"The expression of brain-derived neurotrophic factor (BDNF) mRNA and protein and its primary receptor, TrkB mRNA shows circadian oscillations in adult rats; however it has been unclear if juvenile rats also display a similar pattern in circadian oscillations. We determined the levels of BDNF and TrkB mRNA and of BDNF protein at four separate time points during a 24 hperiod in the hippocampus and frontal cortex. The expression of BDNF and TrkB undergoes diurnal oscillation in adult and postnatal day 21 rats, but no significant variation is present in postnatal day 13 rats. Antidepressant drug treatment also is known to influence BDNF and TrkB levels. However, the reported effects of antidepressant drug treatment on BDNF and TrkB are highly variable and may be influenced by multiple factors, including detection method, class of antidepressant drug, and length of administration. BDNF mRNA levels were decreased significantly in the hippo- campus after acute desipramine (a tricyclic antidepressant) treatment compared to control. BDNF mRNA and protein lev- els, as well as TrkB mRNA levels, were unchanged in adult rats after subchronic and chronic treatment with either de- sipramine or escitalopram (a selective serotonin reuptake inhibitor) and treatment consistent with several reports in the lit- erature. This study defines several important factors that must be taken into account when comparing BDNF and TrkB levels both within and among studies.","PeriodicalId":22907,"journal":{"name":"The Open Pharmacology Journal","volume":"3 1","pages":"1-8"},"PeriodicalIF":0.0000,"publicationDate":"2009-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Open Pharmacology Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2174/1874143600903010001","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

The expression of brain-derived neurotrophic factor (BDNF) mRNA and protein and its primary receptor, TrkB mRNA shows circadian oscillations in adult rats; however it has been unclear if juvenile rats also display a similar pattern in circadian oscillations. We determined the levels of BDNF and TrkB mRNA and of BDNF protein at four separate time points during a 24 hperiod in the hippocampus and frontal cortex. The expression of BDNF and TrkB undergoes diurnal oscillation in adult and postnatal day 21 rats, but no significant variation is present in postnatal day 13 rats. Antidepressant drug treatment also is known to influence BDNF and TrkB levels. However, the reported effects of antidepressant drug treatment on BDNF and TrkB are highly variable and may be influenced by multiple factors, including detection method, class of antidepressant drug, and length of administration. BDNF mRNA levels were decreased significantly in the hippo- campus after acute desipramine (a tricyclic antidepressant) treatment compared to control. BDNF mRNA and protein lev- els, as well as TrkB mRNA levels, were unchanged in adult rats after subchronic and chronic treatment with either de- sipramine or escitalopram (a selective serotonin reuptake inhibitor) and treatment consistent with several reports in the lit- erature. This study defines several important factors that must be taken into account when comparing BDNF and TrkB levels both within and among studies.
抗抑郁药物给药时间和时间对大鼠脑源性神经营养因子和TrkB水平的影响
成年大鼠脑源性神经营养因子(BDNF) mRNA和蛋白及其主要受体TrkB mRNA的表达呈现昼夜振荡;然而,尚不清楚幼年大鼠是否也表现出类似的昼夜节律振荡模式。我们在24小时内测定了海马和额叶皮层中四个不同时间点的BDNF和TrkB mRNA以及BDNF蛋白的水平。BDNF和TrkB的表达在成年大鼠和出生后21天呈昼夜振荡,但在出生后13天无明显变化。抗抑郁药物治疗也会影响BDNF和TrkB水平。然而,报告的抗抑郁药物治疗对BDNF和TrkB的影响是高度可变的,可能受到多种因素的影响,包括检测方法、抗抑郁药物类别和给药时间。与对照组相比,急性地西帕明(一种三环抗抑郁药)治疗后,海马区BDNF mRNA水平显著降低。成年大鼠在接受去西帕明或艾司西酞普兰(一种选择性5 -羟色胺再摄取抑制剂)亚慢性和慢性治疗后,BDNF mRNA和蛋白水平以及TrkB mRNA水平均未发生变化,治疗结果与文献中的几篇报道一致。本研究确定了在比较研究内部和研究之间的BDNF和TrkB水平时必须考虑的几个重要因素。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信