Genetic Diversity and Relationship of Ogye Population in Korea Using 25 Microsatellite Markers

Korean Journal of Poultry Science Pub Date : 2018-09-01 DOI:10.5536/KJPS.2018.45.3.229

H. Roh, Seung-Sook Lee, Jae Yong Han, D. Oh, J. Baek, Sung-Hee Kim, Jun-Heon Lee, Y. Ko, I. Jeon, Seungchang Kim, D. Jeon, Jinwook Lee, Kwan-Woo Kim, C. Cho

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引用次数: 1

Abstract

The aim of this study was to evaluate the genetic diversity and relationships of Ogye populations in Korea. A total of 243 genomic DNA samples from 6 Ogye population (Yeonsan Ogye; YSO, Animal Genetic Resources Research Center Ogye; ARO, Chungbuk Ogye; CBO, Chungnam Ogye; CNO, Gyeongbuk Ogye; GBO, Seoul National University Ogye; SUO) and 3 introduced chicken breeds (Rhode Island Red; RIR, White Leghorn; LG, Cornish; CN) were used. Sizes of 25 microsatellite markers were decided using GeneMapper Software(v 5.0) after analyzing ABI 3130XL. A total of 153 alleles were observed and the range was 2 to 10 per each locus. The mean of expected and observed heterozygosity and PIC (Polymorphism Information Content) value was 0.53, 0.50, 0.46 respectively. The lowest genetic distance (0.073) was observed between YSO and SUO, and the highest distance (0.937) between the RIR and CBO. The results of clustering analysis suggested 3 clusters (ΔK=7.96). Excluding GBO population, 5 Ogye populations (YSO, ARO, CBO, CNO, SUO) were grouped in same cluster with high genetic uniformity (0.990, 0.979, 0.989, 0.994, 0.985 respectively). But GBO population was grouped in cluster 1 with low genetic uniformity (0.340). The results of this study can be use to basic data for the genetic evaluation and management of Ogye populations in Korea. (

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利用25个微卫星标记分析韩国乌溪居群的遗传多样性及亲缘关系

本研究的目的是评价韩国乌溪种群的遗传多样性和亲缘关系。6个Ogye种群(涟山Ogye;Ogye动物遗传资源研究中心;忠北乌溪阿罗;忠南乌溪CBO;庆北Ogye中央军委;首尔大学Ogye GBO;SUO)和3个引进鸡品种(罗德岛红;白里窝那;LG、康沃尔郡的;CN)。分析ABI 3130XL后，采用GeneMapper软件(v 5.0)确定25个微卫星标记的大小。共检测到153个等位基因，每个位点2 ~ 10个。期望杂合度和观测杂合度的平均值分别为0.53、0.50和0.46。YSO与SUO的遗传距离最低(0.073)，RIR与CBO的遗传距离最高(0.937)。聚类分析结果为3类(ΔK=7.96)。除GBO群体外，5个Ogye群体(YSO、ARO、CBO、CNO、SUO)聚在同一聚类中，遗传均匀度分别为0.990、0.979、0.989、0.994、0.985。而GBO群体聚在第1类，遗传均匀性较低(0.340)。本研究结果可为韩国乌溪种群的遗传评价和管理提供基础数据。（

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Korean Journal of Poultry Science

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