In vitro Screening for Antituberculosis activity of Wrightia tinctoria bark and Semicarpus anacardium nuts extracts against Mycobacterium bovis by the Risazurin Microtiter Assay Plate Method

Abstract

ABSTRACT; Plants are well known to be the natural source of variety of drugs since long all over the world. In this regard chemical analysis of Bark of Wrightia tinctoria (WT) and nuts of Semicarpus anacardium (SA) have been done to investigate on anti-tuberculosis activity of these plants. Dried WT bark and SA nuts were successively extracted, with petroleum ether, ethyl acetate, methanol and water, using soxhlet apparatus. All the extracts were screened out in vitro for thier anti-tuberculosis activity against Mycobacterium bovis by Resazurin microtiter assay plate method. Minimum inhibitory concentration (MIC) for all the extracts was determined. Ethyl acetate extract of both plants was found to be more effective followed by petroleum ether, methanol and water extracts. Result sowed the potentiality of plant extracts against the Tuberculosis (TB) agent, which may be an effective mean of treatment in future.