Expression of Estrogen Beta (ERs β) Receptor and Ovarian Histopathology Changes in Rats (Rattus norvegicus) Ovarian Hypofunction Model

Abstract

: One of the reproductive disorders that are often found in breeders in Indonesia is ovarian hypofunction. Ovarian hypofunction is a pathological condition in the ovary that is characterized by a decrease in ovarian function that causes inhibition of folliculogenesis and failure of ovulation. The purpose of this study was to develop ovarian hypofunction animal models through cetrorelix acetate induction and observe their effects on the expression of estrogen beta receptors (Ers β ) and histopathological changes in the ovaries. This study used three groups of female Wistar strains (Rattus norvegicus), with ages 8-10 weeks, and body weight 150-180 grams. The treatments in this study included a control group (KN) without cetrorelix acetate, the first group (P1) was treatment group with an injection of cetrorelix acetate 0.009 mg/kg BW, and the second group (P2) was treatment group with an injection of cetrorelix acetate exposure 0.0135 mg/kg BW. Expression of beta estrogen receptors (Ers β ) in the ovaries was analyzed by immunohistochemical methods, and the data were analyzed using the BNJ test (p <0.05). The ovarian histopathological changes were analyzed by the hematoxylin-eosin (HE) staining method, then analyzed qualitatively. The results of this study indicate that the treatment groups P1 and P2 with GnRH antagonists differ significantly compared to the negative control group. The P2 treatment group had the highest reduction in estrogen receptor expression with Ers β by 92.2%. The result of histopathological in P1 and P2 treatment groups were able to inhibit the development of antral follicles. The conclusion of this study is that cetrorelix acetate as GnRH antagonists can reduce estrogen receptor expression and inhibit folliculogenesis in ovarian histopathology.