ESTROGEN ATTENUATES DIMETHYLHYDRAZINE-INDUCED COLON INJURY IN FEMALE RATS VIA ABROGATION OF OXIDATIVE STRESS AND INFLAMMATIONS

Abstract

: The involvement of estrogen, the female sex hormone, in a variety of gastrointestinal conditions has been documented. We studied the effect of endogenous and exogenous estrogen (estradiol benzoate, 30μg/kg/day S.C) for 8 weeks on early preneoplastic markers induced by the intraperitoneal injection of 1,2-dimethylhydrazine (20 mg/kg) in female rats. Either in sham rats or estradiol benzoate administered animals, estrogen abrogated tumor markers (CA 19.9 and CEA), decreased damage and inflammatory cells infiltration in colon tissue, attenuated oxidative stress markers (MDA, SOD, CAT, and GSH) and inflammatory mediators (IL-6 and IL-10). In conclusion, the estrogen protects against colon injury by reducing precancerous colonic lesions and oxidative stress. The research sheds new light on the therapeutic benefits of estrogen against colon injury in rats. respectively on colon tissues exposed to DMH for 8 consecutive weeks in the presence (4 and 8 th or absence (5th, and and of estrogen. Corresponding histograms of fluorescence intensities of the captured pictures were blotted (1st and 4th column). Quantitative analysis of the fluorescence intensity of protein expression (red color for IL-6 and IL-10) obtained from 5 fields from each mouse section was performed using ImageJ software (B, D, F). Statistical analysis of normally distributed variables were tested by parametric one-way ANOVA followed by post hoc Tukey HDS test for multiple comparisons, The expression was located in colon epithelium.