Evaluation of the Frequency of Enterotoxin A (SEA) and Enterotoxin B (SEB) Genes in Clinical Isolates of Staphylococcus aureus in Rafsanjan, Iran

Afsaneh Mozafarianari, A. Kariminik, M. Tashakori
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引用次数: 1

Abstract

Background: Staphylococcus aureus is one of the most important human pathogens that produces a wide range of toxins and causes various diseases. Staphylococcal enterotoxin is the most common cause of food poisoning. In addition, S. aureus enterotoxins are classified into 18 serotypes A to U based on serological and biological properties. Methods: The samples were isolated from clinical specimens and identified by routine bacteriological methods. The isolated S. aureus was evaluated by polymerase chain reaction (PCR) for the detection of the genes encoding SEA and SEA. Results: Based on the PCR results, 3 isolates possessed the enterotoxins B (SEB) gene while none of them showed enterotoxins A (SEA) gene. Conclusions: The obtained results revealed that the clinical samples might be a potential source of the enterotoxigenic strains of S. aureus.
伊朗拉夫桑詹金黄色葡萄球菌临床分离株肠毒素A (SEA)和肠毒素B (SEB)基因频率的评价
背景:金黄色葡萄球菌是人类最重要的病原体之一,可产生多种毒素并引起多种疾病。葡萄球菌性肠毒素是食物中毒最常见的原因。此外,金黄色葡萄球菌肠毒素根据血清学和生物学特性分为A至U 18种血清型。方法:从临床标本中分离样品,采用常规细菌学方法进行鉴定。采用聚合酶链反应(PCR)对分离的金黄色葡萄球菌进行SEA和SEA编码基因的检测。结果:PCR结果显示,3株分离菌株具有肠毒素B (SEB)基因,未发现肠毒素A (SEA)基因。结论:临床标本可能是产肠毒素金黄色葡萄球菌的潜在来源。
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