A complete view of the Escherichia coli O-antigen biosynthesis gene cluster and the development of molecular-based O-serogrouping methods.

A. Iguchi
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引用次数: 2

Abstract

Much of what we know about Escherichia coli populations and epidemiology is defined at some level by O serogroups. Moreover, in our collective knowledge, outbreak and disease reports and elsewhere, all information of pathogenic E. coli have O serogroup records. O-serogroup diversification shows a strong association with the genetic diversity of O-antigen biosynthesis genes, and O-serogroup-specific sequences can be used as genetic markers for identifying O serogroups. We sequenced all the known O-antigen biosynthesis gene clusters (O-AGCs) from the 184 E. coli defined O serogroups and determined their genetic makeup and diversity. Subsequently, based on a highly detailed analysis of O-AGCs, we developed comprehensive and practical molecular O-serogrouping platforms; PCR-based "E. coli O-genotuping PCR" and in silico-based "SerotypeFinder". These simple and exhaustive systems may integrate microbial typing, genomics and evolutionary analyses.
大肠杆菌o型抗原生物合成基因簇的完整观点和基于分子的o型血清分组方法的发展。
我们对大肠杆菌种群和流行病学的了解在某种程度上是由O血清群定义的。此外,在我们的集体知识、疫情和疾病报告等方面,所有致病性大肠杆菌的信息都有O血清组记录。O-血清群多样性与O抗原生物合成基因的遗传多样性密切相关,O-血清群特异性序列可作为鉴定O-血清群的遗传标记。我们对184个大肠杆菌中所有已知的O抗原生物合成基因簇(O- agcs)进行了测序,确定了它们的基因组成和多样性。随后,在对O-AGCs进行详细分析的基础上,我们开发了全面实用的分子o -血清分型平台;基于PCR的“大肠杆菌o型基因分型PCR”和基于硅的“血清型查找器”。这些简单而详尽的系统可以整合微生物分型、基因组学和进化分析。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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