On-chip manipulating and impedance spectroscopy sensing of single mouse embryo

Abstract

A microfluidic device for single mouse embryo manipulation was fabricated and the impedance spectroscopy of the positioned embryo was detected by the electrodes in the microchannel. Experiment results showed that the impedance of embryo varies when it is at different stage. Also, healthy embryos and development-blocked embryos showed different impedance spectroscopy. Compared with the traditional means of development evaluation in clinic, sensing of impedance is a totally new method for embryo development with the benefit of label free, quantifiable, real-time monitoring and had great potential to be used in development status prediction in clinic.