Larvicidal activity of synthesized silver nanoparticles using isoamyl acetate identified in Annona squamosa leaves against Aedes aegypti and Culex quinquefasciatus

Abstract

Synthesis of silver nanoparticles has the potential to be utilized as a good, eco-friendly approach for the control of mosquito population. In the present study, synthesis of silver nanoparticles using isoamyl acetate isolated from Annona squamosa against mosquito larvae was carried out. The synthesized Ag NPs were characterized by UV, XRD, FTIR and FESEM. The FESEM analyses were clearly spherical and cluster shaped. First to fourth instar mosquito larvae were exposed to varying concentrations of isoamyl acetate and synthesized Ag NPs for 24 h. The highest mortality was observed in synthesized Ag NPs against first to fourth instars of (LC₅₀ = 2.50, 2.78, 3.02, 3.05 μg/ml LC₉₀ = 7.52, 8.34, 9.06, 9.15 μg/ml) and Culex quinquefasciatus (LC₅₀ = 2.75, 3.00, 3.21, 3.48 μg/ml; LC₉₀ = 8.25, 9.01, 9.63, 10.44 μg/ml), respectively. The colloidal solution of silver nanoparticles was found to exhibit mosquito larvicidal activity against dengue and filariasis vector.