Surface plasmon resonance based imaging system and some of its multiple biosensing applications

2003 Conference on Lasers and Electro-Optics Europe (CLEO/Europe 2003) (IEEE Cat. No.03TH8666) Pub Date : 2003-06-22 DOI:10.1109/CLEOE.2003.1313790

E. Maillart, K. Brengel, A. Bleau, D. Capela, T. Livache, A. Roget, T. Soussi, M. Canva, Y. Lévy

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Abstract

Surface plasmon resonance is a sensitive method for detecting biomolecular interactions like DNA-DNA, protein-DNA and protein-protein interactions. The principle of this system is to transform a biochemical signal into an optical signal. This method avoids the need of fluorescent labels because we are measuring precisely the local variation of the optical index caused by the biomolecular interaction. This system has been used for many applications including DNA-DNA, protein-DNA and protein-protein interactions and is efficient for every type of interaction if one of the two reagents can be bound to the gold film. After the optimisation of the interacting conditions, it enlightened the specificity of the interaction of the protein with a double strand target DNA. Results will be presented on the affinity of this protein with its target DNA (which are numerous) and on the relative functionality of mutated p53 proteins.

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基于表面等离子体共振的成像系统及其在生物传感领域的应用

表面等离子体共振是检测DNA-DNA、蛋白质- dna和蛋白质-蛋白质相互作用等生物分子相互作用的灵敏方法。该系统的原理是将生化信号转换为光信号。这种方法避免了荧光标记的需要，因为我们精确地测量了由生物分子相互作用引起的光学指数的局部变化。该系统已用于许多应用，包括DNA-DNA，蛋白质- dna和蛋白质-蛋白质相互作用，并且如果两种试剂中的一种可以结合到金膜上，则对每种类型的相互作用都是有效的。通过对相互作用条件的优化，揭示了蛋白与双链靶DNA相互作用的特异性。结果将呈现在这种蛋白的亲和力与它的靶DNA(这是众多的)和突变的p53蛋白的相对功能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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2003 Conference on Lasers and Electro-Optics Europe (CLEO/Europe 2003) (IEEE Cat. No.03TH8666)

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