Antioxidant system response induced by aluminum in two rice cultivars

Abstract

The antioxidant defense system response was evaluated in two rice cultivars (Oryza sativa L.), Fernandes (CNA-1158) and Maravilha (CNA-6843-1), treated with toxic levels of aluminum. After exposure to aluminum we determined plant growth, H2O2 and O2•- contents, lipid peroxidation, antioxidant enzymes activities and ascorbate and dehydroascorbate contents. Al predominantly accumulated in roots of both cultivars but it reduced root and shoot growth only in the Maravilha cultivar. Treatment with aluminum resulted in a reduction of 84 and 60% in the levels of H2O2 in the roots of the cultivars Maravilha and Fernandes, respectively, and of 26% in the levels of O2•- only in the roots of Fernandes cultivar. Increased lipid peroxidation was observed only in the roots of the Maravilha cultivar. In general, the antioxidant enzyme activities were higher in roots and increased in the presence of aluminum, especially in the Fernandes cultivar. The levels of ascorbate were higher in leaves and increased with aluminum treatment, while dehydroascorbate decreased in roots of both cultivars after aluminum treatment. However, the ascorbate/dehydroascorbate ratio increased in the roots of both cultivars after treatment with aluminum. Ascorbate, dehydroascorbate and ascorbate/dehydroascorbate levels found here point to an efficient regeneration of ascorbate, essential for the homeostasis of cellular metabolites involved in reactive oxygen species removal by rice plants treated with aluminum. Therefore, the higher tolerance of Fernandes to aluminum relative to Maravilha cultivar may be the result of better growth of the root system and shoots, higher antioxidant enzyme activities and a best use/regeneration of ascorbate.