Larval Amphibian Growth and Development Assay (LAGDA) (OECD TG 241)

Abstract

422. Modality detected/endpoints: OECD TG 241 has three endpoints indicating generalised toxicity (mortality, abnormal behaviour and growth), and several providing specific information about endocrine disruption or impaired reproduction (histopathology of thyroid, gonads, kidney and liver, time to metamorphosis [NF stage 62]; secondary sex characteristics (nuptial pads); vitellogenin (optional); genetic and phenotypic sex ratio). Most of these specific endocrine endpoints are likely to respond to interference with the hypothalamic/pituitary/gonadal (HPG) axis, while thyroid histopathology and time to metamorphosis may respond to interference with the hypothalamic/pituitary/thyroid axis (as may the “generalised toxicity” indicator, growth).