Anti-inflammatory effect of non-thermal atmospheric pressure plasma for periodontitis treatment: in vitro pilot study

You li Park, Hyun-Joo Kim, Ju-Youn Lee, Sung-Hee Jeong, Eun-Young Kwon, Ji-Young Joo
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引用次数: 1

Abstract

Purpose: The purpose of this study was to evaluate the anti-inflammatory effects of non-thermal atmospheric pressure plasma (NTP) on human gingival fibroblasts (HGFs) for clinical application of periodontal treatment. Materials and Methods: HGFs were treated with Porphyromonas gingivalis (Pg) lipopolysaccharide (LPS). Customized NTP device was developed for periodontal in vitro study. Cell viability was evaluated with cell counting kit-8. The levels of inflammatory cytokines, including interleukin (IL)-8 and 6, were determined by enzyme-linked immunosorbent assay. Results: When NTP was applied, the cell viability did not change significantly, and there was no difference for 6 h and 24h. When Pg LPS was treated to HGFs, the secretion of IL-8 and IL-6 was increased compared to the control group. But when the NTP was applied, the secretion of them was significantly decreased. Conclusion: NTP did not affect cell viability of HGFs. And it inhibited the LPS-induced production of IL-8 and IL-6. (J Dent Rehabil Appl Sci 2021;37(2):88-94)
非热常压血浆治疗牙周炎的抗炎作用:体外初步研究
目的:研究非热常压血浆(NTP)对人牙龈成纤维细胞(HGFs)的抗炎作用,为牙周治疗的临床应用提供依据。材料与方法:采用牙龈卟啉单胞菌(Pg)脂多糖(LPS)处理hgf。为牙周体外研究量身定制NTP装置。用细胞计数试剂盒-8测定细胞活力。用酶联免疫吸附法测定炎症细胞因子,包括白细胞介素(IL)-8和6的水平。结果:NTP作用后,细胞活力无明显变化,6 h和24h无明显差异。当Pg LPS作用于HGFs时,与对照组相比,IL-8和IL-6的分泌增加。但应用NTP后,它们的分泌明显减少。结论:NTP对hgf细胞活力无影响。抑制lps诱导的IL-8和IL-6的产生。[J] .康复与应用科学,2021;37(2):88-94。
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