In vitro and in silico study to evaluate the effectiveness of quercitrin as antiviral drug to dengue virus

THE 4TH BIOMEDICAL ENGINEERING’S RECENT PROGRESS IN BIOMATERIALS, DRUGS DEVELOPMENT, HEALTH, AND MEDICAL DEVICES: Proceedings of the International Symposium of Biomedical Engineering (ISBE) 2019 Pub Date : 2019-12-10 DOI:10.1063/1.5139341

B. Dewi, E. Ratningpoeti, H. Desti, M. Angelina

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引用次数: 7

Abstract

Dengue Fever (DF) in Indonesia has been a major health problem for the last 47 years, which keeps on rising until today. Quercitrin as one of the natural compounds, flavonol group and has been used in several types of research for their properties of anti-inflammation, anti-bacterial, anti-viral, and anti-fungi. The aim of this study was to explore the potency of quercitrin as an antiviral drug to dengue virus (DENV) in vitro and in silico. We used Focus assay, MTT assay, and docking analysis to determine IC50, CC50 and binding energy, respectively. The IC50, CC50 and Selectivity Index (SI) of quercitrin was 1.1 µg/ml, 38.8 µg/ml and 38 respectively. In silico study showed the binding energy between quercitrin and NS5 protein was −7,54 kkal/mol. The results obtained that Quercutrin as a good candidate for an antiviral drug to DENV in the future.Dengue Fever (DF) in Indonesia has been a major health problem for the last 47 years, which keeps on rising until today. Quercitrin as one of the natural compounds, flavonol group and has been used in several types of research for their properties of anti-inflammation, anti-bacterial, anti-viral, and anti-fungi. The aim of this study was to explore the potency of quercitrin as an antiviral drug to dengue virus (DENV) in vitro and in silico. We used Focus assay, MTT assay, and docking analysis to determine IC50, CC50 and binding energy, respectively. The IC50, CC50 and Selectivity Index (SI) of quercitrin was 1.1 µg/ml, 38.8 µg/ml and 38 respectively. In silico study showed the binding energy between quercitrin and NS5 protein was −7,54 kkal/mol. The results obtained that Quercutrin as a good candidate for an antiviral drug to DENV in the future.

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体外和计算机研究评价槲皮素作为登革热病毒抗病毒药物的有效性

过去47年来，登革热在印度尼西亚一直是一个主要的健康问题，直到今天仍在不断上升。槲皮素作为黄酮醇类天然化合物之一，因其具有抗炎、抗菌、抗病毒、抗真菌等特性而被广泛应用于多种研究中。本研究的目的是探讨槲皮素作为登革热病毒(DENV)的抗病毒药物在体外和体内的效力。采用Focus法、MTT法和对接法分别测定IC50、CC50和结合能。槲皮苷的IC50、CC50和选择性指数分别为1.1µg/ml、38.8µg/ml和38。结果表明，槲皮素与NS5蛋白的结合能为−7.54 kkal/mol。结果表明，槲皮素是未来抗DENV的良好候选药物。过去47年来，登革热在印度尼西亚一直是一个主要的健康问题，直到今天仍在不断上升。槲皮素作为黄酮醇类天然化合物之一，因其具有抗炎、抗菌、抗病毒、抗真菌等特性而被广泛应用于多种研究中。本研究的目的是探讨槲皮素作为登革热病毒(DENV)的抗病毒药物在体外和体内的效力。采用Focus法、MTT法和对接法分别测定IC50、CC50和结合能。槲皮苷的IC50、CC50和选择性指数分别为1.1µg/ml、38.8µg/ml和38。结果表明，槲皮素与NS5蛋白的结合能为−7.54 kkal/mol。结果表明，槲皮素是未来抗DENV的良好候选药物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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THE 4TH BIOMEDICAL ENGINEERING’S RECENT PROGRESS IN BIOMATERIALS, DRUGS DEVELOPMENT, HEALTH, AND MEDICAL DEVICES: Proceedings of the International Symposium of Biomedical Engineering (ISBE) 2019

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